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( C ) Protein levels of SREBP-1c, SCD1, and CHAC1 in livers of mice under HFD

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This research was supported by the National Natural Science Foundation of China (grant number 82101089), the Henan Province Youth Health Science and Technology Innovation Talent Training Program (grant number YQRC2024004), the Henan Science and Technology R&D Program Joint Fund Project (grant number 235200810064), the Henan Medical Science and Technology Talent Overseas Training Program (grant number H20240040), the Basic Science Project of Henan Eye Institute/Henan Eye Hospital (grant number 23JCZD002), the Provincial and Ministry Co-constructed Key Projects of the Henan Provincial Health Commission (grant number SBGJ202302098), and the Young Medical Researchers Program of Henan Academy of Medical Sciences (grant number QNYJ2023014)

Absorption considerations are important when determining appropriate dosage

To stop the reaction at specific time-points, 10 L of each reaction was incubated with 10 L of 2X stopping buffer (1 % formic acid / 10 % acetonitrile), spun down, and kept on ice